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How Do You Get Exosomes?

Home » Knowledge Center » Exosomes » How Do You Get Exosomes?

How Do You Get Exosomes?
exosomes, method, exosome, therapy, exosomal, extraction, ultracentrifugation, hair, skin, growth

While exosomal therapy continues to increase in popularity, many want to know where exosomes come from and how they are safely harvested and administered. In the past, exosomes were thought to be metabolic waste and of no use in our body. However, when it was discovered that exosomes play vital roles in our bodies, many in the medical research community began to sit up and take notice.

As research about exosomes continues, we find their importance increase within our bodies. For example, we only recently discovered that exosomes can be helpful in the early diagnosis of several degenerative diseases and can also be helpful in treating ED in men. Researchers have also discovered that they can assist in carrying molecules containing medication or other chemicals to target specific areas during treatment.

How is an Exosome Isolated and Extracted?

Generally, there are five exosome isolation and extraction methods. Each method follows a unique exosome trait, that aids the process of extracting exosomes from the bodily fluids and cells that produce them. The following are the methods that are used in the isolation and extraction of exosomes.

  • Ultracentrifugation – The most widely used exosome extraction method. It is popular in exosome extraction because it is a straightforward method. However, while it is the most efficient method, it is also the most demanding one because of the time commitment and equipment necessary to run successfully. It’s mainly done in two forms, differential ultracentrifugation and the density gradient ultracentrifugation.

Density gradient ultracentrifugation is done by mainly focusing on the density of the exosome. Through this method, exosomes are harvested through ultracentrifugation based on density.

In differential ultracentrifugation, the exosomes are isolated and extracted according to their size. The centrifugal force has to be small, because a sizable centrifugal force may end up damaging the exosomal structure.

  • Ultrafiltration – This method also uses the size characteristic of an exosome to isolate and extract it. Generally, it is a relatively simple and efficient method because it does not interfere with the biological activities of the exosomes during the extraction. However, there is also a possibility of collecting other vesicles of similar size, reducing the purity of the extracted solution.
  • Polypolymer Precipitation – This method uses the principle of precipitation when isolating exosomes. The precipitation process is easy to operate, and consists of only a short cycle.
  • Antibody affinity purification – This method uses a magnetic bead method to isolate and extract exosomes. It is an efficient process because it has high specificity, and its operation is relatively simple. Moreover, the exosomes extracted through this method, contain fewer impurities compared to other methods but can be labor and time intensive.
  • Aqueous two-phase system – This method extracts exosomes by taking advantage of the aqueous phase incompatibility of molecules. It applies the liquid fractionation technique to isolate the exosomes.

All these exosome isolation and extraction methods have the limitation of impurity. Due to this, further isolation and purification of the initial exosomal solution is often necessary. Those resulting exosomes are then used for treating different conditions such as erectile dysfunction (ED), hair loss and joint pain among many others.

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ED Therapy with Exosomes

When used as part of a medically supervised treatment plan, exosomal therapy can not only help correct ED, for most men it also can help effectively address the underlying causes of erectile dysfunction. At HealthGAINS, our medical team utilizes many cutting edge therapies as part of each patient’s personalized treatment plan. For men suffering from ED, exosomes can typically be used in concert with GAINSwave which itself stimulates the production and release of growth factors within the penis. The result is more frequency, more satisfying erections and orgasms for most men.

Exosomes Therapy For Hair Loss

Purified exosomes are essential growth factors that have been proven to help in hair regeneration and growth. Therefore, they are useful in hair restoration treatments. Under a doctor’s close supervision, exosomal therapy is administered to encourage hair growth hormones in the body.

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Exosomal Skin Therapy

This therapy works by using exosomes to address many of the issues associated with the aesthetic beauty of our skin. Typically, patients have exosomes administered using a microneedling technique that has been shown to drastically improve the look, feel and elasticity of the skin.

Another type of exosomal skin therapy uses an ultrasound that produces heat to drive exosomes deeper into the skin to help reduce the signs of age like scars, age lines and wrinkles.

At HealthGAINS, we are committed to helping our patients achieve their personalized goals with individualized plans. The key to unlocking your personal goals is personalized plans, attention and therapies like those found at our clinic. Call us today to get started or to find out more about how exosomal therapy can help you live a longer, more fulfilled life.

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Exosomes FAQ:

Why are Exosomes so Important
Why are Exosomes so Important

The role that exosomal cells, which are sometimes referred to as blebs, minicells or microvesicles, play in transmitting cellular information within the body has become one of the most exciting areas of medical research in recent years. These tiny vesicles facilitate communication from one cell to the other and are ideal for the delivery of ...

What Is Exosome Therapy?
What Is Exosome Therapy?

Living with degenerative conditions and chronic disease is not something that anyone wishes to experience. If you are one of the millions of Americans that suffer from a chronic disease or illness, you likely know that finding solutions to manage your health can be an extremely frustrating experience. Often we find ourselves looking for alternative ...

What Is Exosome Therapy?
What Is Exosome Therapy?

Living with degenerative conditions and chronic disease is not something that anyone wishes to experience. If you are one of the millions of Americans that suffer from a chronic disease or illness, you likely know that finding solutions to manage your health can be an extremely frustrating experience. Often we find ourselves looking for alternative ...

What are Exosomes?
What are Exosomes?

Exosomes are tiny vesicles, packaged in molecules that are released by cells in the body. They have been shown to be the primary mode of moving information between cells. They play a large role in the transportation of proteins, lipids and nucleic acids between cells in the body. They are produced throughout the body and ...

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